Owi Taq Master Mix Ready To Load is an optimised 2X premix containing DNA polymerase, dNTPs, MgCl2, KCl, stabilisers, and a loading dye. Only template DNA and primers need to be added. Compatible with purified DNA, bacterial colonies, liquid cultures, crude extracts, or cDNA, it offers great versatility. It allows amplification up to 10kb from complex genomic DNA (e.g., human) and up to 20kb from simple templates (e.g., lambda phage). Its standard extension time is 30s/kb and can be reduced to 5s/kb for fast PCR. Ideal for standard PCR, colony PCR for construct identification, and direct PCR for genotyping.
- Versatile: compatible with all species
- Robust: tolerance to PCR inhibitors
- Efficient: performs on GC-/AT-rich regions
- Direct-colony PCR: no DNA extraction required
- Fast: amplification from 5 sec/kb
- Ready-to-load: includes loading dye for direct gel analysis
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